TECHNICAL EXPERTISE
-
Eukaryotic cell lineages and primary cultures (CEFs, MEFs, and PBMCs, including monocyte-derived DCs).
-
In vitro functional assays for cell biology, immunology and virology
-
Generation of modified cell lines by transfection/electroporation, lentiviral vectors or CRISPR-Cas9.
Cell culture
Virology
-
Manipulation of BSL-2/-3 viruses:
- Flavivirus, Alphavirus, Herpesviridae, Orthomyxoviridae, Birnaviridae, Enterovirus, and others) .
​
-
General techniques such as virus infection, titration, purification, and others.
-
General molecular biology techniques and assays.
- Extraction/purification, cloning, sequencing, vector building
​
-
RNA manipulation/modification and secondary structure analysis (SHAPE).
Molecular Biology
Serology techniques
-
Classical complement fixation and neutralization assays.
-
Large experience with the development of ELISAs and lateral flow immunochromatographic assays (rapid tests).
-
Confocal microscopy and Live-cell imaging
-
High-content imaging system (Operetta CLS™, PerkinElmer).
-
Multicolour flow cytometry and sorting.
Bioimaging
Others
-
Generation of monoclonal antibodies by hybridoma technology.
-
Antibody characterization, purification and conjugation.
-
Small-scale production of antibodies, recombinant protein and virus-like particles (VLPs) in prokaryotic and eukaryotic systems.
-
Experienced in field/mobile BSL-3 work.
TRANSFERABLE SKILLS
-
Writing scientific papers and projects for personal funding and lab grants.
-
Oral presentation skills of science projects/results to experts or laypeople
​
-
Languages:
- Portuguese (native)
- English (fluent)
- French (fluent)
- Spanish (advanced)
- Italian (intermediate)
- Korean (beginner)
Written & Oral Communication
Mentoring and Teaching
-
Co-supervisor of undergraduate, MSC, and PhD students.
-
Lecturer of Molecular Biology, Biotechnology Technician Program. SENAI-PR, Brazil (2013-2015).
Project Management & Organization
-
Development of intra- and inter-institutional collaborative projects
-
Project management
-
Laboratory management
-
Ability to carry out multiple simultaneous projects.