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TECHNICAL EXPERTISE

  • Eukaryotic cell lineages and primary cultures (CEFs, MEFs, and PBMCs, including monocyte-derived DCs).

 

  • In vitro functional assays for cell biology, immunology and virology

 

  • Generation of modified cell lines by transfection/electroporation, lentiviral vectors or CRISPR-Cas9.

Cell culture

Virology

  • Manipulation of  BSL-2/-3 viruses: 

- Flavivirus, Alphavirus, Herpesviridae, Orthomyxoviridae, Birnaviridae, Enterovirus, and others) .

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  • General techniques such as virus infection, titration, purification, and others.

  • General molecular biology techniques and assays.

- Extraction/purification, cloning, sequencing, vector building 

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  • RNA manipulation/modification and secondary structure analysis (SHAPE).

Molecular Biology

Serology techniques

  • Classical complement fixation and neutralization assays.

 

  • Large experience with the development of ELISAs and lateral flow immunochromatographic assays (rapid tests).

  • Confocal microscopy and Live-cell imaging

 

  • High-content imaging system (Operetta CLS™, PerkinElmer).

 

  • Multicolour flow cytometry and sorting.

Bioimaging

Others

  • Generation of monoclonal antibodies by hybridoma technology.

 

  • Antibody characterization, purification and conjugation.

 

  • Small-scale production of antibodies, recombinant protein and virus-like particles (VLPs) in prokaryotic and eukaryotic systems.

 

  • Experienced in field/mobile BSL-3 work.

TRANSFERABLE SKILLS

  • Writing scientific papers and projects for personal funding and lab grants.

 

  • Oral presentation skills of science projects/results to experts or laypeople 

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  • Languages:

 - Portuguese (native)

 - English (fluent)

 - French (fluent)

 - Spanish (advanced)

 - Italian (intermediate)

 - Korean (beginner)

Written & Oral Communication

Mentoring and Teaching

  • Co-supervisor of undergraduate, MSC, and PhD students.

 

  • Lecturer of Molecular Biology, Biotechnology Technician Program. SENAI-PR, Brazil (2013-2015).

Project Management & Organization

  • Development of intra- and inter-institutional collaborative projects

 

  • Project management

 

  • Laboratory management

 

  • Ability to carry out multiple simultaneous projects.

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